Theme 3: Nanodiscs and Lipid-Based Mimetics for Structural Biology

Creating and benchmarking membrane mimetics that retain native lipid interactions and support advanced structural methods.

The laboratory actively develops and contributes to evaluate detergent-based nanodiscs as bilayer mimetics. These efforts provide insights into lipid composition, protein insertion, functional preservation, and suitability for cryo-EM, NMR, or SAXS. The work includes both methodological advances and applications to challenging targets such as TRPC channels.

Associated publications

• Utilizing Native Nanodiscs to Isolate Active TRPC3 Channels and Expand Structural Analysis Capabilities. Scientific Reports 2025. (DOI: 10.1038/s41598-025-13218-6)
• Home Is Where the Lipids Are: Comparing MSP and DDDG Nanodiscs for Membrane Protein Research. Soft Matter 2025. (DOI: 10.1039/D5SM00327J)
• Self-Assembly of Protein-Containing Lipid-Bilayer Nanodiscs from Small-Molecule Amphiphiles. Small 2021. (DOI: 10.1002/smll.202103603)